Students supported by The Bashan Foundation



Luis Leyva and Claudia Rojas are constructing an
anti-herbivorous metal fence in a field experiment
using native desert trees to protect the newly planted seedlings (2004).
Name: Luis A. Leyva
Degree: M.Sc. in: Use,management and conservation of natural resources
Years: 2003-2006
Graduate school: The Center for Biological Research of the Northwest, La Paz, Mexico
Location of thesis’ work: The Center for Biological Research of the Northwest, La Paz, Mexico
Thesis: Effect of gluconic acid on its metabolic enzymes in the plant growth-promoting bacterium Azospirillum brasilense Cd associated with mesquite trees. .
Type of support: Financial and scientific advising
Publication from thesis:
  • Leyva L.A. and Bashan, Y. 2008. Activity of two catabolic enzymes of the phosphogluconate pathway in mesquite roots inoculated with Azospirillum brasilense Cd. Plant Physiology and Biochemistry 46 : 898-904

Luis Leyva is helping to eradicate an invasive grass
in CIBNOR field preserve to make room for
re-vegetation with native plants (2003).

Name: Adan Trejo
Degree: Diploma in Biology
Years: 2005-2006
Graduate school: The Autonomus Metropolitan University (UAM), Xochimilco, Mexico City, Mexico
Location of thesis’ work: The Center for Biological Research of the Northwest, La Paz, Mexico
Thesis: Characterization of Chlorella sorokiniana LAP1, a heat and solar-radiation tolerant microalgae, for wastewater treatment under extreme desert conditions.
Type of support: Financial and scientific advising.
Publication from thesis:
  • de-Bashan, L.E., Trejo, A., Huss, V.A.R., Hernandez, J.-P. Bashan, Y. 2008. Chlorella sorokiniana UTEX 2805, a heat and intense, sunlight-tolerant microalga with potential for removing ammonium from wastewater. Bioresource Technology 99: 4980-4989


Juan–Pablo Hernandez (left) and Adan Trejo (right) evaluating a high light intensity- high temperature photobioreactor for wastewater treatment with microalgae.

wastewater, phototrophic, chlorella
Octavio analyzing dissolved oxygen in some
samples of wastewater from the wastewater
treatment plant of La Paz, Mexico.
Name: Octavio Perez
Degree: M.Sc. in: Use,management and conservation of natural resources
Years: 2006-2009
Graduate school: The Center for Biological Research of the Northwest, La Paz, Mexico
Location of thesis’ work: The Center for Biological Research of the Northwest, La Paz, Mexico
Thesis: Heterotrophic growth of Chlorella vulgaris coimmobilized with Azospirillum brasilense in alginate beads to remove nitrogen and phosphorus from wastewater.
Type of support: Financial and scientific advising
Publications from thesis:
  • Perez-Garcia, O., de-Bashan, L.E., Hernandez, J.-P., and Bashan, Y. 2010. Efficiency of growth and nutrient uptake from wastewater by heterotrophic, autotrophic, and mixotrophic cultivation of Chlorella vulgaris immobilized with Azospirillum brasilense. Journal of Phycology 46: 800–812

  • Perez-Garcia, O., Escalante, F.M.E., de-Bashan L.E., and Bashan, Y. 2011. Heterotrophic cultures of microalgae: Metabolism and potential products. Water Research 45: 11-36. The definitive version is available at: http://www.elsevier.com

  • Perez-Garcia, O., Bashan, Y., and Puente M.E. 2011. Organic carbon supplementation of sterilized municipal wastewater is essential for heterotrophic growth and removing ammonium by the microalga Chlorella vulgaris. Journal of Phycology 47: 190-199.

Name: Blanca Estela Romero Lopez
Degree: D.Sc. in: Use,management and conservation of natural resources
Years: 2004-2009
Graduate school: The Center for Biological Research of the Northwest, La Paz, Mexico
Location of thesis’ work: The Center for Biological Research of the Northwest, La Paz, Mexico
Thesis: Effect of plant-endophytes interaction on rock weathering.
Type of support: Financial and scientific advising
Publications from thesis:
 acetylene, mammillaria
Blanca injecting acetylene to her mammilaria
samples to determine nitrogen fixation.

chlorella, FISH, DGGE, restoration
Adan preparing a gel for PCR.
Name: Adan Trejo
Degree: M.Sc. in: Use,management and conservation of natural resources
Years: 2007-2010
Graduate school: The Center for Biological Research of the Northwest, La Paz, Mexico
Location of thesis’ work: The Center for Biological Research of the Northwest, La Paz, Mexico
Thesis: The potential use of microbiological residues from biological wastewater treatment; their application as inoculant for improvement of eroded soils.
Type of support: Financial and scientific advising
Publications from thesis:
  • Trejo, A., de-Bashan, L. E., Hartmann, A., Hernandez, J.P., Rothballer, M., Schmid, M. and Bashan, Y. 2012. Recycling waste debris of immobilized microalgae and plant growth-promoting bacteria from wastewater treatment as a resource to improve fertility of eroded desert soil. Environmental and Experimental Botany 75 : 65-73

  • Lopez, B.R., Bashan, Y., Trejo, A., and de-Bashan, L.E. 2013. Amendment of degraded desert soil with wastewater debris containing immobilized Chlorella sorokiniana and Azospirillum brasilense significantly modifies soil bacterial community structure, diversity, and richness. Biology and Fertility of Soils 49: 1053-1063


Name: Sergio Hernandez Covarrubias
Degree: M.Sc. in: Use,management and conservation of natural resources
Years: 2009-2011
Graduate school: The Center for Biological Research of the Northwest, La Paz, Mexico
Location of thesis’ work: The Center for Biological Research of the Northwest, La Paz, Mexico
Thesis: Interaction of the jointly immobilized microalga-bacteria system with the microbial community of domestic wastewater .
Type of support: Financial and scientific advising
Publication from thesis:
Sergio Hernandez Covarrubias
Sergio is thinking about his
next year and next experiments
with microalgae.

mangrove, restored, Balandra
Alejandra Vovides is measuring salinity in
the restored mangrove ecosystem at Balandra
Lagoon, near the city of La Paz,
Baja California Sur, Mexico.
Name: Alejandra Vovides
Degree: D.Sc. in: Use,management and conservation of natural resources
Years: 2006-2011
Graduate school: Ecology Institute, Xalapa, Mexico
Location of thesis’ work: The Center for Biological Research of the Northwest, La Paz, Mexico
Thesis: Nitrogen fixation in disturbed, reforested and conserved mangroves in arid and tropical zones of Mexico.
Type of support: Financial and scientific advising
Publications from thesis:
  • Vovides, A. G., Bashan, Y., Lopez-Portillo, J. A. and Guevara, R. 2011 Nitrogen fixation in preserved, reforested, naturally regenerated and impaired mangroves as an indicator of functional restoration in mangroves in an arid region of Mexico. Restoration Ecology 19: 236–244

  • Vovides, A.G., Lopez-Portillo, J., and Bashan, Y. 2011. N2-fixation along a gradient of long-term disturbance in tropical mangroves bordering the gulf of Mexico. Biology and Fertility of Soils 47: 567–576


Name: Ivonne Cruz Snatander
Degree: M.Sc. in: Use,management and conservation of natural resources
Years: 2010-2012
Graduate school: The Center for Biological Research of the Northwest, La Paz, Mexico
Location of thesis’ work: The Center for Biological Research of the Northwest, La Paz, Mexico
Thesis: Evaluation in situ of the immobilized system Chlorella vulgaris-Azospirillum brasilense during tertiary wastewater treatment.
Type of support: Financial and scientific advising
Publication from thesis:
  • Cruz, I., Bashan, Y., Hernandez-Carmona, G., and de-Bashan, L.E. 2013. Biological deterioration of alginate beads containing immobilized microalgae and bacteria during tertiary wastewater treatment. Applied Microbiology and Biotechnology 97: 9847-9858

  • ivonne cruz santander

    Ivonne Cruz measuring phosphorus and ammonium obtained from a photobioreactor and heterotrophic bioreactor treating wastewater with the microalgae Chlorella vulagris.

    Beatriz meza
    Name: Beatriz Meza Marquez
    Degree: M.Sc. in: Use,management and conservation of natural resources
    Years: 2010-2013
    Graduate school: The Center for Biological Research of the Northwest, La Paz, Mexico
    Location of thesis’ work: The Center for Biological Research of the Northwest, La Paz, Mexico
    Thesis: Effect of indole-3-acetic-acid-producing bacterium Azospirillum brasilense on the microalga Chlorella vulgaris related to ammonium removal from synthetic wastewater treatment.
    Type of support: Financial and scientific advising
    Publications from thesis:
  • Meza, B., de-Bashan, L. E., and Bashan, Y. 2015 Involvement of indole-3-acetic acid produced by Azospirillum brasilense in accumulating intracellular ammonium in Chlorella vulgaris. Research in Microbiology 166: 72-83

  • Meza, B., de-Bashan, L. E., Hernandez, J. P., and Bashan, Y. 2015 Accumulation of intra-cellular polyphosphate in Chlorella vulgaris cells is related to indole-3-acetic acid produced by Azospirillum brasilense. Research in Microbiology 166: 399-407


  • Name: Francisco Javier Choix Ley
    Degree: D.Sc. in: Use,management and conservation of natural resources
    Years: 2010-2014
    Graduate school: The Center for Biological Research of the Northwest, La Paz, Mexico
    Location of thesis’ work: The Center for Biological Research of the Northwest, La Paz, Mexico
    Thesis: Evaluation of accumulation of carbohydrates in the microalgae Chlorella spp. associated with the bacterium Azospirillum brasilense
    Type of support: Financial and scientific advising
    Publications from thesis:
    • Palacios, O.A., Choix, F.J., Bashan, Y, de-Bashan, L.E. 2016. Influence of tryptophan ans indole-3-acetic acid on starch accumulation in the synthetic mutualistic Chlorella sorokinianaAzospirillum brasilense system under heterotrophic conditions. Research in Microbiology. 167:367-379.

    • Choix, F.J., Bashan, Y., Mendoza, A., and de-Bashan, L.E. 2014. Enhanced activity of ADP glucose pyrophosphorylase and formation of starch induced by Azospirillum brasilense in Chlorella vulgaris. Journal of Biotechnology 177: 22-34

    • Choix, F. J., de-Bashan, L.E. and Bashan, Y. 2012 Enhanced accumulation of starch and total carbohydrates in alginate-immobilized Chlorella spp. induced by Azospirillum brasilense: I. Autotrophic conditions. Enzyme and Microbial Technology 51: 294–299

    • Choix, F. J., de-Bashan, L.E. and Bashan, Y. 2012 Enhanced accumulation of starch and total carbohydrates in alginate-immobilized Chlorella spp. induced by Azospirillum brasilense: II. Heterotrophic conditions. Enzyme and Microbial Technology 51: 300–309

    francisco javier choix ley

    Francisco Choix is measuring pH and accumulation of carbohydrates in the microalga Chlorella vulgaris coimmobilized with Azospirillum brasilense


    Name: Emmanuel Vidana Avila
    Degree: M.Sc. in: Use,management and conservation of natural resources
    Years: 2011-2014
    Graduate school: The Center for Biological Research of the Northwest, La Paz, Mexico
    Location of thesis’ work: The Center for Biological Research of the Northwest, La Paz, Mexico
    Thesis: Effect of glycerol as carbon source in the immobilized system of microalgae-bacteria under heterotrophic conditions for removing ammonium and phosphate.
    Type of support: Financial and scientific advising
    Publication from thesis:
    emmanunel vidaña


    edgar amavizca

    Name: Edgar Amavizca Valdez
    Degree: M.Sc. in: Use,management and conservation of natural resources
    years: 2011-2014
    Graduate school: The Center for Biological Research of the Northwest, La Paz, Mexico
    Location of thesis’ work: The Center for Biological Research of the Northwest, La Paz, Mexico
    Thesis: Evaluation of remote effects of plant growth-promoting bacteria on the microalgae Chlorella vulgaris
    Type of support: Financial and scientific advising
    Publication from thesis:

    Name: Luis Alonso Leyva
    Degree: D.Sc. in: Use,management and conservation of natural resources
    Years: 2009-2014
    Graduate school: The Center for Biological Research of the Northwest, La Paz, Mexico
    Location of thesis’ work: The Center for Biological Research of the Northwest, La Paz, Mexico
    Thesis: Accumulation of lipids and fatty acids in the microalgae Chlorella vulgaris co-immobilized in alginate beads with Azospirillum brasilense in relation to cultivation conditions.
    Type of support: Financial and scientific advising
    Publications from thesis:
  • Leyva, L.A., Bashan Y., Mendoza, A., and de-Bashan, L.E. 2014. Accumulation of fatty acids in Chlorella vulgaris under heterotrophic conditions in relation to activity of acetyl-CoA carboxylase, temperature, and co-immobilization with Azospirillum brasilense. Naturwissenschaften 101:819–830

  • Leyva, L.A., Bashan Y., and de-Bashan, L.E. 2015. Activity of acetyl-CoA carboxylase is not directly linked to accumulation of lipids when Chlorella vulgaris is co-immobilised with Azospirillum brasilense in alginate under autotrophic and heterotrophic conditions. Annals of Microbiology 65: 339-349

  • Luis Alonso Leyva

    Luis is preparing raw-fish cocktail (ceviche) for the traditional Christmas party .


    Cristina galviz

    Name: Cristina Galaviz Bustamante
    Degree: M.Sc. in: Use,management and conservation of natural resources
    Years: 2012-2015
    Graduate school: The Center for Biological Research of the Northwest, La Paz, Mexico
    Location of thesis’ work: The Center for Biological Research of the Northwest, La Paz, Mexico
    Thesis: Changes in soil fertility and in the bacterial community of the rhizosphere of mesquite after inoculation with plant growth-promoting bacteria aimed to restore eroded desert soils.
    Type of support: Financial and scientific advising
    Publication from thesis:

    Name: Edisa Garcia
    Degree: M.Sc. in: Use,management and conservation of natural resources
    Years: 2012-2015
    Graduate school: The Center for Biological Research of the Northwest, La Paz, Mexico
    Location of thesis’ work: The Center for Biological Research of the Northwest, La Paz, Mexico
    Thesis: Study of the structure and physiological profile of the soil bacterial community in the mesquite (Prosopis articulata) resource islands in relation with the plant diversity
    Type of support: Financial and scientific advising
    Publication from thesis:
    Edisa Garcia


    Oskar

    Oskar is preparing his experiments on the effect of vitamins on A. brasilense

    Name: Oskar Palacios Lopez
    Degree: D.Sc. in: Use,management and conservation of natural resources
    Years: 2011-2015
    Graduate school: The Center for Biological Research of the Northwest, La Paz, Mexico
    Location of thesis’ work: The Center for Biological Research of the Northwest, La Paz, Mexico
    Thesis: Study of microalgae-bacteria interaction on thiamine and tryptophan production by Chlorella sorokiniana and their effect on IAA biosynthesis by Azospirillum brasilense.
    Type of support: Financial and scientific advising
    Publications from thesis:
  • Palacios, O.A., Choix, F.J., Bashan, Y, de-Bashan, L.E. 2016. Influence of tryptophan ans indole-3-acetic acid on starch accumulation in the synthetic mutualistic Chlorella sorokinianaAzospirillum brasilense system under heterotrophic conditions. Research in Microbiology. 167:367-379.

  • Palacios, O.A., Bashan, Y., and de-Bashan, L.E. 2014. Proven and potential involvement of vitamins in interactions of plants with plant growth-promoting bacteria—an overview. Biology and Fertility of Soils 50: 415-432

  • Palacios, O. A., Bashan, Y., Schmid, M., Hartmann, A., de-Bashan, L. E. 2016. Enhancement of thiamine release during synthetic mutualism between Chlorella sorokiniana and Azospirillum brasilense growing under stress conditions. Journal of Applied Phycology. 28:1521–1531.


  • students, mangrove, cactus