Dr. Luz de Bashan and Dr. Yoav Bashan
Genetic manipulation and molecular detection and monitoring
of plant growth-promoting bacteria (Basic studies)
Version January 2016
Goals: Genetically manipulate Plant Growth-Promoting Bacteria (PGPB);
production of Azospirillum strains with reporter genes (Green Fluorescent Protein). Detection of PGPB by molecular
technique such as Fluorescense in situ Hybridization (FISH) and monitoring microbial populations using Denaturing
Gradient Gel Electrophoresis (DGGE).
For More PDF files of earlier papers go to: Environmental Microbiology
Mendoza, Centro de Biotecnología Genómica (CBG), Reynosa, Tamaulipas,
Dr. Gracia Alicia Gómez Anduro,
Center for Biological Research of the Northwest,, La Paz, Mexico
Most relevant publications
de-Bashan, L.E., Hernandez, J.-P., Bashan, Y. and Maier, R. 2010. Bacillus pumilus ES4: Candidate plant growth-promoting bacterium to enhance
establishment of plants in mine tailings.
Environmental and Experimental Botany 69: 343–352
de-Bashan, L.E., Hernandez, J.-P., Nelson, N. K., Bashan, Y. and Maier, R. 2010. Growth of Quailbush in Acidic, Metalliferous Desert Mine Tailings: Effect of Azospirillum brasilense Sp6 on Biomass Production and Rhizosphere Community Structure. Microbial Ecology 60: 915–927
Rodriguez, H., Fraga, T. and Bashan, Y.
2006. Genetics of phosphate solubilization and potential applications for improving plant growth-promoting bacteria.
Plant and Soil 287: 15 - 21.
Rodriguez, H., Mendoza, A., Cruz, M.A., Holguin, G., Glick B.R., and Bashan, Y. 2006.
Pleiotropic physiological effects in the plant growth-promoting bacterium Azospirillum brasilense following chromosomal labeling in the clpX gene.
FEMS Microbiology Ecology 57: 217-225.
Bacilio, M., Rodriguez H., Moreno, M., Hernandez,
J.-P., and Bashan Y. 2004. Mitigation of salt stress in wheat seedlings by a gfp-tagged
Azospirillum lipoferum. Biology and
Fertility of Soils 40: 188-193.