Dr. Yoav Bashan, Dr. Luz de Bashan
and M. Sc. Juan-Pablo Hernandez

Water bioremediation and advanced wastewater treatments
(Applied studies)

Version January 2016

Goal: Develop concepts and technologies on how to recycle used water (wastewater) in arid lands with unique combinations of microalgae and microalgae growth-promoting bacteria.

For More PDF files of earlier papers go to: Environmental Microbiology Website.

External Collaborators

  • Ing. Abigail Solano, La Paz Wastewater Treatment Facility, La Paz, Mexico.
beads, microalgae, bacteria, azospirillum, chlorella
The history of how the microalgae-plant growth promoting bacteria model
was born
click on the imagen

Bioreactors for experimental wastewater treatment containing immobilized microalgae (inside polymeric gels) growing under mixotrophic conditions (light and dark periods).

Noga Bashan (L) and Ivonne Cruz (R) measuring growth parameters in a photobioreactor with domestic wastewater containing immobilized microalgae.

Most relevant publications in recent years:

  • Meza, B., de-Bashan, L.E., and Bashan, Y. 2015. Involvement of indole-3-acetic acid produced by Azospirillum brasilense in accumulating intracellular ammonium in Chlorella vulgaris. Research in Microbiology 166: 72-83

  • Meza, B., de-Bashan, L. E., Hernandez, J. P., and Bashan, Y. 2015 Accumulation of intra-cellular polyphosphate in Chlorella vulgaris cells is related to indole-3-acetic acid produced by Azospirillum brasilense. Research in Microbiology 166: 399-407

  • Perez-Garcia, O., and Bashan, Y. 2015. Microalgal heterotrophic and mixotrophic culturing for bio-refining: From metabolic routes to techno-economics. Algal Biorefineries. Vol. 2: Products and Refinery Design. Prokop, A., Bajpai, R., Zappi, M. (Eds). Springer International Publishing Switzerland, pp. 61-131

  • Leyva, L.A., Bashan Y., Mendoza, A., and de-Bashan, L.E. 2014. Accumulation of fatty acids in Chlorella vulgaris under heterotrophic conditions in relation to activity of acetyl-CoA carboxylase, temperature, and co-immobilization with Azospirillum brasilense. Naturwissenschaften 101:819–830

  • Choix, F.J., Bashan, Y., Mendoza, A., and de-Bashan, L.E. 2014. Enhanced activity of ADP glucose pyrophosphorylase and formation of starch induced by Azospirillum brasilense in Chlorella vulgaris. Journal of Biotechnology 177: 22-34

  • Leyva, L.A., Bashan Y., and de-Bashan, L.E. 2014. Activity of acetyl-CoA carboxylase is not directly linked to accumulation of lipids when Chlorella vulgaris is co-immobilised with Azospirillum brasilense in alginate under autotrophic and heterotrophic conditions. Annals of Microbiology (in press) DOI 10.1007/s 13213-014-0866-3

  • Cruz, I., Bashan, Y., Hernandez-Carmona, G., and de-Bashan, L.E. 2013. Biological deterioration of alginate beads containing immobilized microalgae and bacteria during tertiary wastewater treatment. Applied Microbiology and Biotechnology 97: 9847-9858

  • Lopez, B.R., Bashan, Y., Trejo, A., and de-Bashan, L.E. 2013. Amendment of degraded desert soil with wastewater debris containing immobilized Chlorella sorokiniana and Azospirillum brasilense significantly modifies soil bacterial community structure, diversity, and richness. Biology and Fertility of Soils 49: 1053-1063

  • Trejo, A., de-Bashan, L. E., Hartmann, A., Hernandez, J.P., Rothballer, M., Schmid, M. and Bashan, Y. 2012. Recycling waste debris of immobilized microalgae and plant growth-promoting bacteria from wastewater treatment as a resource to improve fertility of eroded desert soil. Environmental and Experimental Botany 75 : 65-73

  • Covarrubias, S.A., de-Bashan, L.E., Moreno, M., and Bashan, Y. 2012. Alginate beads provide a beneficial physical barrier against native microorganisms in wastewater treated with immobilized bacteria and microalgae. Applied Microbiology and Biotechnology 93: 2669-2680
    The definitive version is available electronically on SpringerLink: http://www.springerlink.com/openurl.asp?genre=article&id=doi:10.1007/s00253-011-3585-8

  • Perez-Garcia, O., Bashan, Y., and Puente M.E. 2011. Organic carbon supplementation of sterilized municipal wastewater is essential for heterotrophic growth and removing ammonium by the microalga Chlorella vulgaris. Journal of Phycology 47: 190-199.

  • Perez-Garcia, O., de-Bashan, L.E., Hernandez, J.-P., and Bashan, Y. 2010. Efficiency of growth and nutrient uptake from wastewater by heterotrophic, autotrophic, and mixotrophic cultivation of Chlorella vulgaris immobilized with Azospirillum brasilense. Journal of Phycology 46: 800–812

  • de-Bashan, L.E. and Bashan, Y. 2010 Immobilized microalgae for removing pollutants: Review of practical aspects. Bioresource Technology 101: 1611-1627

  • de-Bashan, L.E., Trejo, A., Huss, V.A.R., Hernandez, J.-P. Bashan, Y. 2008. Chlorella sorokiniana UTEX 2805, a heat and intense, sunlight-tolerant microalga with potential for removing ammonium from wastewater. Bioresource Technology 99: 4980-4989

  • Yabur, R., Bashan Y., Hernández-Carmona G. 2007. Alginate from the macroalgae Sargassum sinicola as a novel source for microbial immobilization material in wastewater treatment and plant growth promotion. Journal of Applied Phycology 19: 43-53.

  • Hernandez, J-P., de-Bashan, L.E., and Bashan, Y. 2006. Starvation enhances phosphorus removal from wastewater by the microalgae Chlorella spp.co-immobilized with Azospirillum brasilense. Enzyme and Microbial Technology 38: 190-198

  • de-Bashan L.E., Hernandez J.-P., Morey, T., and Bashan, Y. 2004. Microalgae growth-promoting bacteria as "helpers" for microalgae: a novel approach for removing ammonium and phosphorus for municipal wastewater. Water Research 38:466-474.
  • de-Bashan, L.E. and Bashan, Y. 2004. Recent advances in removing phosphorus from wastewater and its future use as fertilizer (1997-2003). Water Research 38: 4222-4246
  • Valderrama, L.T., Del Campo, C.M., Rodriguez, C.M., de-Bashan, L.E., and Bashan, Y. 2002. Treatment of recalcitrant wastewater from ethanol and citric acid production using the microalgae Chlorella vulgaris and the macrophyte Lemna minuscule. Water Research 36: 4185-4192
  • de-Bashan, L.E., Moreno, M., Hernandez, J.P., and Bashan, Y. 2002. Removal of ammonium and phosphorus ions from syntetic waste water by the microalgae Chlorella vulgaris coimmobilized in alginate beads with the microalgae growth-promoting bacterium Azospirillum brasilense . Water Research 36: 2941-2948.
  • Growth under extreme conditions. Growth of the wastewater treatment microalgae (Chorella sorokiniana) in high light intensity photoreactor.

    Photosynthetic microalga (Chorella vulgaris) immobilized in polymer beads growing under autotrophic (light) condition in inverted bioreactors for wastewater treatment.

    Related pages:

  • Device for producing large-sized polymer beads

  • A method for automated fast production of large-sized polymer beads (2–4 mm)



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