Dr. Yoav Bashan, Dr. Luz de Bashan
and M. Sc. Juan-Pablo Hernandez

Bacterial inoculants and mixed inoculants to enhance plant growth (applied studies)

Version January 2016

Goal: Invent practical, advanced inoculants (a.k.a “bio-fertilizers”) for improving growth of agricultural and forest plants.


Bacterial inoculant made of alginate beads mixed with wheat seeds.

For More PDF files of earlier papers go to: Environmental Microbiology Website.

External Collaborators


Most relevant publications in the recent years:

  • Bashan, Y., and de-Bashan, L. E. 2015 Inoculant Preparation and Formulations for Azospirillum spp. In: Handbook for Azospirillum (pp. 469-485). Springer International Publishing

  • Bashan, Y., de-Bashan, L.E., Prabhu, S.R., and Hernandez, J.-P. 2014. Advances in plant growth-promoting bacterial inoculant technology: formulations and practical perspectives (1998-2013). (A Marschner Review). Plant and Soil 378: 1-33

  • Bashan, Y., Trejo, A. and de-Bashan, L.E. 2011. Development of two culture media for mass cultivation of Azospirillum spp. and for production of inoculants to enhance plant growth. Biology Fertility of Soil 47: 963-969

  • Puente, M.E., Li, C.Y. and Bashan, Y. 2009 Endophytic bacteria in cacti seeds can improve the development of cactus seedlings. Environmental and Experimental Botany 66: 402-408

  • Yabur, R., Bashan Y., Hernández-Carmona G. 2007. Alginate from the macroalgae Sargassum sinicola as a novel source for microbial immobilization material in wastewater treatment and plant growth promotion. Journal of Applied Phycology 19: 43-53.

  • Bashan, Y., Hernandez, J.P., Leyva, L.A. and Bacilio, M. 2002. Alginate microbeads as inoculant carrier for plant growth-promoting bacteria. Biology and Fertility of Soils 35: 359-368.

  • Rojas, A., Holguin, G., Glick, B.R. and Bashan, Y. 2001. Synergism between Phyllobacterium sp. (N2-fixer) and Bacillus licheniformis (P-solubilizer), both from a semiarid mangrove rhizosphere. FEMS Microbiology Ecology 35 :181-187

  • Bashan, Y. and Gonzalez, L.E. 1999. Long-term survival of the plant-growth-promoting bacteria Azospirillum brasilense and Pseudomonas fluorescens in dry alginate inoculant. Applied Microbiology and Biotechnology 51 : 262-266.

  • Bashan, Y. 1998. Inoculants of plant growth-promoting bacteria for use in agriculture. Biotechnology Advances 16: 729-770.

alginato de sargassum
Alginate beads containing the Plant growth-promotign bacteria Azospirillum brasilense Cd. above beads from Macrocystis pyrifera below beads from Sargassum sinicola (Inoculant for agriculture)
diferentes esferas de alginato
Different beads of alginate from various sources. (from left to rigth: control of Macrocystis pyrifera, control of Sargassum sinicola, immobilized of Macrocystis pyrifera (Chlorella), immobilized of Sargassum sinicola (Chlorella), co-immobilized of Macrocystis pyrifera (Chlorella with Azospirillum), co-immobilized of Sargassum sinicola. (Chlorella with Azospirillum))

Personal page of Yoav Bashan

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