Dr. Yoav Bashan, Dr. Luz de Bashan,
M. Sc. Juan-Pablo Hernandez and M. Sc. Manuel Moreno

Inoculation of cultivated and wild plants with plant growth-promoting bacteria (basic studies).

Version January 2016

Goals: Understand how plant growth by plant growth-promoting bacteria might affect the yield of agricultural plants for better agricultural programs and establish wild plants to improve reforestation programs.

cactus cardon azospirillum
Manuel Moreno is removing seeds from the fruit of Cardon cactus (Pachycereus pringlei)
cactus cardon azospirillum
Seedlings of giant cardon cactus growing in
crushed extrusive igneous rock after one year
(left: inoculated with Bacillus subtilis, center:
non-inoculated, right: fertilized.)
cactus cardon azospirillum
Reforestation of desert lands: two years after planting blue palo verde (native tree) and cardon cactus growing together
cactus cardon azospirillum
General view of the reforested desert three
years after planting with Mesquite amargo
cactus cardon azospirillum Restored desert area with blue palo verde two years after planting.

For More PDF files of earlier papers go to: Environmental Microbiology Website.

Most relevant publications in recent years:

  • Bashan, Y., de-Bashan, L.E., Prabhu, S.R., and Hernandez, J.-P. 2014. Advances in plant growth-promoting bacterial inoculant technology: formulations and practical perspectives (1998-2013). (A Marschner Review). Plant and Soil 378: 1-33

  • Palacios, O.A., Bashan, Y., and de-Bashan, L.E. 2014. Proven and potential involvement of vitamins in interactions of plants with plant growth-promoting bacteria—an overview. Biology and Fertility of Soils 50: 415-432

  • Bashan, Y., Kamnev, A.A., de-Bashan, L.E. 2013 Tricalcium phosphate is inappropriate as a universal selection factor for isolating and testing phosphate-solubilizing bacteria that enhance plant growth: a proposal for an alternative procedure. Biology and Fertility of Soils 49: 465-479 DOI: 10.1007/s00374-012-0737-7.

  • Lopez, B.R., Bashan, Y., Trejo, A., and de-Bashan, L.E. 2013. Amendment of degraded desert soil with wastewater debris containing immobilized Chlorella sorokiniana and Azospirillum brasilense significantly modifies soil bacterial community structure, diversity, and richness. Biology and Fertility of Soils 49: 1053-1063

  • Bashan, Y., Kamnev, A.A., and de-Bashan, L.E. 2013. A proposal for isolating and testing phosphate-solubilizing bacteria that enhance plant growth. Biology and Fertility of Soils 49: 1-2.

  • Bashan, Y., Salazar, B. G., Moreno M., Lopez, B. R. and Linderman, R. G. 2012 Restoration of eroded soil in the Sonoran Desert with native leguminous trees using plant growth-promoting microorganisms and limited amounts of compost and water. Journal of Environmental Management 102 : 26-36

  • Lopez, B.R., Tinoco-Ojanguren, C., Bacilio, M., Mendoza, A., and Bashan, Y., 2012. Endophytic bacteria of the rock-dwelling cactus Mammillaria fraileana affect plant growth and mobilization of elements from rocks. Environmental and Experimental Botany 81: 26-36

  • Bashan, Y., Trejo, A. and de-Bashan, L.E. 2011. Development of two culture media for mass cultivation of Azospirillum spp. and for production of inoculants to enhance plant growth. Biology Fertility of Soil 47: 963-969

  • Puente, M.E., Li, C.Y. and Bashan, Y. 2009 Endophytic bacteria in cacti seeds can improve the development of cactus seedlings. Environmental and Experimental Botany 66: 402-408

  • Bashan, Y., Salazar, B., and Puente, M.E. 2009. Responses of native legume desert trees used for reforestation in the Sonoran Desert to plant growth-promoting microorganisms in screen house. Biology and Fertility of Soils 45:655-662 The definitive version is available at: http://www.springerlink.com

  • Bashan, Y., Salazar, B., Puente, M. E., Bacilio, M., and Linderman, R.G. 2009. Enhanced establishment and growth of giant cardon cactus in an eroded field in the Sonoran Desert using native legume trees as nurse plants aided by plant growth-promoting microorganisms and compost. Biology and Fertility of Soils 45: 585-594 The definitive version is available at: http://www.springerlink.com

  • Leyva L.A. and Bashan, Y. 2008. Activity of two catabolic enzymes of the phosphogluconate pathway in mesquite roots inoculated with Azospirillum brasilense Cd. Plant Physiology and Biochemistry 46 : 898-904

  • Bacilio M., Hernandez, J.-P., and Bashan, Y. 2006. Restoration of giant cardon cacti in barren desert soil amended with common compost and inoculated with Azospirillum brasilense. Biology and Fertility of Soils 43: 112-119

  • Bashan, Y., Bustillos, J.J., Leyva, L.A., Hernandez, J-P., and Bacilio M. 2006. Increase in auxiliary photoprotective photosynthetic pigments in wheat seedlings induced by Azospirillum brasilense. Biology and Fertility of Soils 42: 279-285

  • Bashan, Y., and de-Bashan, L.E. 2005. Bacteria/Plant growth-promotion. In: Encyclopedia of soils in the environment. (Ed.) D. Hillel, Elsevier, Oxford, U.K.. Volume 1. pp.103-115.

  • Bashan, Y. and de-Bashan, L.E. 2005. Fresh-weigth measurements of roots provide inaccurate estimates of the effects of plant growth-promoting bacteria on root growth: a critical examination. Soil Biology & Biochemistry 37: 1795-1804

  • Bashan, Y., Holguin, G. and de-Bashan, L.E. 2004. Azospirillum-plant relationships: physiological, molecular, agricultural, and enviromental advances (1997 - 2003). Canadian Journal of Microbiology 50: 521 - 577

  • Bacilio M., Rodriguez H., Moreno M., Hernandez J-P., and Bashan Y. 2004. Mitigation of salt stress in wheat seedlings by a gfp- tagged Azospirillum lipoferum. Biology and Fertility of Soil 40: 188-193

  • Bacilio M., Vazquez P., and Bashan Y. 2003. Alleviation of noxious effects of cattle ranch composts on wheat seed germination by inoculation with Azospirillum spp. Biology and Fertility of Soils 38: 261-266.

  • Bashan, Y. and de-Bashan, L.E. 2002. Reduction of bacterial speck (Pseudomonas syringae pv tomato) of tomato by combined treatments of plant growth-promoting bacterium, Azospirillum brasilense, streptomycin sulfate, and chemo-thermal seed treatment. European Journal of Plant Pathology 108: 821-829.

  • Bashan, Y., Hernandez, J.P., Leyva, L.A. and Bacilio, M. 2002. Alginate microbeads as inoculant carrier for plant growth-promoting bacteria. Biology and Fertility of Soils 35: 359-368.

  • Carrillo, A.E., Li, C.Y., and Bashan, Y. 2002. Increased acidification in the rhizosphere of cactus seedlings induced by Azospirillum brasilense. Naturwissenschaften 89: 428-432.

  • Bashan, Y., and de-Bashan, L.E. 2002. Protection of tomato seedlings against infection by Pseudomonas syringae pv tomato using the plant growth-promoting bacterium Azospirillum brasilense . Applied and Environmental Microbiology 68: 2637-2643.


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